SOIL SURVEY LABORATORY METHODS MANUAL by USDA
By USDA
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1986. Sampling. In A. ) Methods of soil analysis. Part 1. Physical and mineralogical methods. 2nd ed. Agronomy 9:33-51. 21 SAMPLE COLLECTION AND PREPARATION LABORATORY PREPARATION OF SOIL SAMPLES (1B) WHOLE SOIL (1B6) 1. APPLICATION Coarse fragments and fine-earth material are homogenized so that analyses can be made on the whole soil basis. The preparation code for this procedure is "GP". Refer to Appendix I, Laboratory Preparation Codes. Entire sample is crushed to <2 mm, and analytical results are reported on whole soil basis.
Weight measurements are made on the 20to 75-mm, 5- to 20-mm, and 2- to 5-mm fractions. The <2-mm material is saved for chemical, physical, and mineralogical analysis. 3. INTERFERENCES Soil variability and sample size are interferences to sample collection and preparation. At each stage of sampling, an additional component of variability, the variability among smaller elements within the larger units, is added to the sampling error (Petersen and Calvin, 1986). Soil material needs to be in adequate amount and thoroughly mixed to obtain a representative sample.
The line on each bottle is 13 cm which is the distance from the center of rotation to the surface of the suspension. Stopper and shake well to mix the suspension. 3 Balance opposite centrifuge loads, which consist of centrifuge bottle, trunnion carrier and bucket. Place loads on a torsion balance and add water to the lighter bucket until both loads weigh the same. 4 Read the temperature of the suspension. 5 Centrifuge at 1500 rpm. 2 µm) (3A1b) r = 18 cm Nm = 1500 rpm. 6 After centrifuging, lower the pipet to a 3-cm depth in the suspension.