API RP 1166 Excavation Monitoring and Observation by API
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Example text
N N N The great similarity in properties of oxyhemoglobin and carbonmonoxyhemoglobin provides strong support for this structure. The structure in which each of the two oxygen atoms (connected with one another by a single bond) is attached to the iron atom by a single bond is rendered improbable by the strain involved in the three-membered ring. —In contrast to oxyhemoglobin and carbonmonoxyhemoglobin, hemoglobin itself contains unpaired electrons, its magnetic susceptibility showing the presence of a pronounced paramagnetic contribution.
In the first series a portion of solution A was made more and more acid by the addition, with rapid stirring, of successive small portions of 1 N hydrochloric acid solution, values of pH and Aw being determined after each acidification. These data are given in Table III and shown by the horizontally barred circles in Fig. 1. The formation of a small amount of coagulum in the acid solution was noticed; in order to eliminate possible error due to this coagulation, the following series of measurements was made.
23, respectively. The contribution of hemoglobin to the ionic strength was ignored. The data for these solutions represent a typical titration curve, the molal susceptibility changing rapidly over the range pH. 5 from an asymptotic value of about 14,000-10 ~6, representing ferrihemoglobin, Hb+, to an asymptotic value of about 830040 -6 , representing ferrihemoglobin hydroxide, HbOH. Using these estimated asymptotes to calculate concentrations of Hb+ and HbOH, it is found that the experimental points when plotted on a graph of log ([Hb+]/[HbOH]) against pH.