By W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)
This quantity contains 20 contributions of the twelfth assembly on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. a number of fields of ultracentrifugation are coated touching on examine difficulties in biochemistry, biophysical chemistry and macromolecular chemistry in addition to interacting platforms. New investigations about the sedimentation concept are offered. The section transition of gels is handled, as is the sedimentation-diffusion equilibrium of gels. One part includes the hydrodynamics of biopolymers.
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Additional resources for Analytical Ultracentrifugation VI
18], a commercial analytical ultracentrifuge equipped with absorption optics (as also done in Refs. [13, 31]), combined with a recently introduced linear least-squares method for the calculation of an apparent sedimentation coeﬃcient distribution, was applied. The latter method is based on boundary modelling by a superposition of sedimentation proﬁles of ideal nondiﬀusing particles . Our approach certainly lacks the high-throughput capability of the noncommercial systems and lacks the capability of permitting the high-resolution analysis of extremely broad size distributions in a single run, as oﬀered by the ‘‘gravitational sweep method’’ [17, 30].
Academic, New York 21. Staﬀord WF (1992) Anal Biochem 203:295 22. Schuck P (2000) Biophys J 78:1606 23. Svedberg T, Pedersen KO (1940) Die Ultrazentrifuge. Steinkopﬀ, Dresden 24. Mie G (1908) Ann Phys 25:377 25.
Precup Æ C. H. Weidl U. S. Schubert Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, 5600 MD Eindhoven, The Netherlands P. Schuck Division of Bioengineering and Physical Science, ORS, National Institutes of Health, Bethesda, MD 20892-5766, USA H. Durchschlag Institut fu¨r Biophysik und Physikalische Biochemie, Universita¨t Regensburg, 93040 Regensburg, Germany W. Ma¨chtle Kunststoﬄaboratorium, BASF AG, 67056 Ludwigshafen, Germany POLYMERS, COLLOIDS AND SUPRAMOLECULAR SYSTEMS Studies on the partial specific volume of a poly(ethylene glycol) derivative in different solvent systems Abstract The speciﬁc volume of charged supramolecular compounds dissolved in organic solvents varies considerably with the solvent system applied; in addition, it is inﬂuenced by the presence of salt.