Textbook of Vitreoretinal Diseases and Surgery by S. Natarajan
By S. Natarajan
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Identification of photoreceptor precursors in the pars plana during ocular development and after retinal injury. Invest Ophthalmol Vis Sci 2008; 49 (1): 422-8. 30. Nickerson PE, Emsley JG, Myers T, Clarke DB. Proliferation and expression of progenitor and mature retinal phenotypes in the adult mammalian ciliary body after retinal ganglion cell injury. Invest Ophthalmol Vis Sci 2007; 48 (11): 5266-75. 31. Jomary C, Jones SE. Induction of functional photoreceptor phenotype by exogenous Crx expression in mouse retinal stem cells.
52. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH. Viable offspring derived from fetal and adult mammalian cells. Nature 1997; 385 (6619): 810-3. 53. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126 (4): 663-76. 54. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007; 448 (7151): 313-7. 55. Arnhold S, Klein H, Semkova I, Addicks K, Schraermeyer U.
The cells were also able to integrate into degenerating mouse retina. 42, 44 The studies mentioned above use fetal calf serum or fetal tissues as feeder layers to direct differentiation of ES cells into photoreceptors. The differentiation medium used here is not defined and would involve use of xenogenic agents and hence may not be suitable for transplantation in human patients. 49 The authors used a combination of purified signaling molecules in a step-wise manner and combined this with selection of cells based on progenitor-specific markers at each step to finally generate photoreceptors from ES cells.