Protein Synthesis DNA Synthesis and Repair RNA Synthesis by Paul D. Boyer (Eds.)
By Paul D. Boyer (Eds.)
Read Online or Download Protein Synthesis DNA Synthesis and Repair RNA Synthesis Energy-Linked ATPases Synthetases PDF
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Additional resources for Protein Synthesis DNA Synthesis and Repair RNA Synthesis Energy-Linked ATPases Synthetases
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Szer, JBC (1974) (in press). 1. IROSOMALBINDINGO F fMgT Factor additions Experiment Temp. 5 From Chae el al. (54). 5 A l W units). Incubation 15 min. c Net values (blanks with no added factors subtracted) in picomoles per sample. 0 b TABLE V AUG-DEPENDENT BINDING OF fMET t,ltNA WITH RInosoMAL 0' AND 2 5 " " ~ ~ N SUBUNITS A T Factor additions (pg) Temp. 0 From Chae et al. (64). 6 A*@units) were used. As assayed by poly U-dependent polyphenylalanine synthesis the contamination of 30 S with 50 S subunits waa about 10%.
111. Eucaryotes A. INITIATOR AMxNoACYL-tRNA Although the analysis of protein biosynthesis with use of cell-free systems began with preparations from eucaryotic organisms, mainly with rat liver and reticulocytes, the study of eucaryotic protein synthesis was overshadowed for scvcral years by the use of systems derived from bacteria, mostly from E. coli. I n recent years the mechanism of protein biosynthesis in mammalian cells, plant cells, and cells of other eucaryotic organisms has been gaining increased attention, the main questions being the similarities and differences in the mechanisms of protein biosynthesis between procaryotes and eucaryotes.
99. D. A. Shafritz and W. F. Anderson, Nature (London) 227, 918 (1970). 100. D. A. Shafritz, D. G. Laycock, nnd W. F. Anderson, Proc. N a t . Acntl. Sci. 68,496 (1971). 101. A. Shafritz, D. G . Laycock, R. C. Crystal, and W. F. Anderson, Proc. N n t . Acad. Sci. 68,2246 (1971). 102. A. Shafritz, P. M. Prichard, J. M. Gilbert, W. C. Merrick, and W. IZ, Anderson, Proc. Nut. Acad. Sci. 69,983 (1972). 1. 27 From Shafritz et al. (101). Incubations were performed for 3 min at 23". 0 pmoles of substrate was used: E.