Molecular Genetic Approaches to Maize Improvement by Alan L. Kriz, Brian A. Larkins

Molecular Genetic Approaches to Maize Improvement by Alan L. Kriz, Brian A. Larkins

By Alan L. Kriz, Brian A. Larkins

Prior to now decade, there was large growth in maize biotechnology. This quantity offers an outline of our present wisdom of maize molecular genetics, the way it is getting used to enhance the crop, and destiny probabilities for crop enhancement. numerous chapters take care of genetically engineered characteristics which are presently, or quickly may be, in advertisement construction. Technical techniques for introducing novel genes into the maize genome, the regeneration of vegetation from remodeled cells, and the construction of transgenic strains for box construction are lined. additional, the authors describe how molecular genetic suggestions are getting used to spot genes and represent their functionality, and the way those approaches are applied to increase elite maize germplasm. additionally, molecular biology and physiological reports of corn as a foundation for the advance of its dietary and food-making homes are incorporated. ultimately, the becoming use of corn as biomass for strength construction is mentioned.

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Additional resources for Molecular Genetic Approaches to Maize Improvement (Biotechnology in Agriculture and Forestry, Volume 63)

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Plant J 44:693–705 Wu L, Nandi S, Chen L, Rodriguez RL, Huang N (2002) Expression and inheritance of nine transgenes in rice. Transgenic Res 11:533–541 Yu W, Han F, Gao Z, Vega JM, Birchler JA (2007) Construction and behavior of engineered minichromosomes in maize. ) plants. Theor Appl Genet 107:1157–1168 Zhao Z-Y, Gu W, Cai T, Tagliani L, Hondred D, Bond D, Schroeder S, Rudert M, Pierce D (2001) High throughput genetic transformation of maize mediated by Agrobacterium tumefaciens. Mol Breed 8:323–333 Zhong H, Srinivasan C, Sticklen MB (1992) In-vitro morphogenesis of corn Zea mays L.

An interesting sidebar to protoplast transformation was to deliver DNA molecules to intact, cell-wall-encased maize cells via electroporation (D’Halluin et al. 1992). While initially promising, this technique proved to be technically challenging and, ultimately, inefficient and was never widely adopted. 2 Particle Bombardment (Biolistics) The development of particle bombardment technology (“biolistics”) was a significant development in crop transformation that eliminated the need to transfer DNA into naked plant protoplasts (Klein et al.

ZFNs can, therefore, be theoretically targeted to virtually any site in the genome. Wright et al. (2005) demonstrated the feasibility of this approach by targeting a partially deleted, inactive GUS:NPT II fusion gene in a specific site into tobacco protoplasts that contained the missing sequence from the fusion gene. Homologous recombination restored the function of the GUS:NPT II genes. In this set of experiments, targeted integration was observed 10% of the time, an increase in homologous recombination of four to five orders of magnitude (Wright et al.

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