Biotechnology in Plant Science. Relevance to Agriculture in by Milton Zaitlin

Biotechnology in Plant Science. Relevance to Agriculture in by Milton Zaitlin

By Milton Zaitlin

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Additional resources for Biotechnology in Plant Science. Relevance to Agriculture in the Eighties

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A. (1978). New Phytol. 80, 517. V. (1983). , Vol. 1, 82. M. (1976). Analytical Biochem. 72^, 248. Browers, M . A . , and Orton T. J. (1982). Plant Sei. Lett. 2 6 , 65. L. (1982). Planta 1 5 6 , 520. Esau, K. (1965) "Plant Anatomy", J. Wiley and Sons, N . Y . , pp. 318. Evans, D . A . , Sharp, W. E. (1981), In "Plant Tissue Culture" (Thorpe, T . A . Y. , p . 45. , Thorpe, T . A . K. Vasil (1976). In vitro 12, 473. Halperin, W. (1964). Science 1 4 6 , 408. A. (1967). J. Ultrastruct. Res. 18, 428.

RESPONSE OF REGENERATING CELLS TO CHANGES IN THE REGENERATION MEDIUM Embryos form when alfalfa callus is induced on 2,4-D contain­ ing medium and transferred onto hormone-free medium containing the necessary components. When cells are transferred to fresh regeneration medium at 2 days, however, embryo formation is prevented (Fig. 2 and 3). In studies done on carrot and soybean, it has been shown that cells plated onto hormone-free medium will release 2,4-D into the medium (Montague £t al_. , 1981).

It is suggested in the example of Tripo­ gandra referred to above that the vascular connections to the 2 types of anthers are different and that those anthers containing S pollen have a restricted and limiting nutrient supply which means the pollen is effectively starved of substances necessary to ensure normal development. It is possible that there is a Embryogenesis from Pollen In Vitro 55 Fig. 2. Pollen dimorphism in Hordeum vulgare cv. Sabarlis. (a) Normal mature starch-filled grain with atypical S grain in which the single nucleus has divided symmetrically.

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