By Lokesh Bhattacharyya, Jeffrey S. Rohrer
This can be a complete resource of data at the program of ion chromatography (IC) within the research of pharmaceutical medicinal drugs and biologicals. This ebook, with members from academia, pharma, the biotech undefined, and device production, offers different views, adventure, and services of the idea leaders of IC in a entire demeanour. It explores strength IC functions in numerous points of product improvement and qc checking out. furthermore, an appendix part provides info on serious actual and chromatographic parameters on the topic of IC and data on present brands of IC platforms, columns, and different parts.
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Additional info for Applications of Ion Chromatography in the Analysis of Pharmaceutical and Biological Products
J. Chromatogr. A 1998;818:61–68. 31. Novic M, Haddad PR. Indirect UV spectrophotometric detection in Ion Exclusion Chromatography—Insights into retention processes, presented at the International Ion Chromatography Symposium, 2003 September 21-24 2003, paper B-4; San Diego, CA. REFERENCES 47 32. Tanaka K, Ding MY, Helaleh MIH, Taoda H, Takahashi H,. Hu WZ, Hasebe K, Haddad PR, Fritz JS, Sarzanini C. Vacancy ion-exclusion chromatography of carboxylic acids on a weakly acidic cation-exchange resin.
31. Novic M, Haddad PR. Indirect UV spectrophotometric detection in Ion Exclusion Chromatography—Insights into retention processes, presented at the International Ion Chromatography Symposium, 2003 September 21-24 2003, paper B-4; San Diego, CA. REFERENCES 47 32. Tanaka K, Ding MY, Helaleh MIH, Taoda H, Takahashi H,. Hu WZ, Hasebe K, Haddad PR, Fritz JS, Sarzanini C. Vacancy ion-exclusion chromatography of carboxylic acids on a weakly acidic cation-exchange resin. J. Chromatogr. A 2002;956(1–2):209–214.
The more acidic the eluent, the more protonation occurs as the ionized analyte diffuses from the occluded phase and this in turn increases the propensity for the analyte to re-enter the pore and leads to increased retention of the analyte. In summary, acidic eluents stimulate strong diffusion of the protonated analyte into the pores at the leading edge of the analyte band. Combined with the decreased intensity of its back-repulsion into the interstitial eluent at the tailing edge of the analyte band, this produces both symmetrical peaks and increased retention times.